2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis Isoform Specific Products:

DNA/RNA Synthesis Isoform Comparison
Cat. No. Product Name Effect Purity Chemical Structure
  • HY-W001974
    7-Nitroindole
    		99.88%
    7-Nitroindole is a cleavable base analog and Antibacterial agent. 7-Nitroindole inhibits the growth of Lactobacillus arabinosus. 7-Nitroindole is used in studies of light-controlled DNA cleavage and Lactobacillus arabinosus infection.
    7-Nitroindole
  • HY-16031B
    AFP464 dihydrochloride
    		Inhibitor 99.67%
    AFP464 (NSC710464) dihydrochloride is a prodrug of Aminoflavone (HY-132974) and an agonist of the aryl hydrocarbon receptor (AhR). AFP464 dihydrochloride downregulates the expression of α6-integrin (α6-integrin), inhibits breast tumor growth, reduces the population of tumor-initiating cells, disrupts mammosphere structure, induces the formation of mucin lake clusters, triggers DNA damage, and exerts antiproliferative activity. AFP464 dihydrochloride is rapidly converted to Aminoflavone by nonspecific esterases in plasma and cell culture media. AFP464 dihydrochloride is applicable to research related to breast cancer.
    AFP464 dihydrochloride
  • HY-180176A
    ALC1-IN-1 free base
    		Inhibitor
    ALC1-IN-1 (compound 1) free base is an ALC1 inhibitor in crystalline form that can be used in the research of cancer.
    ALC1-IN-1 free base
  • HY-N0007AR
    Bisdemethoxycucurmin (Standard)
    		Inhibitor
    Bisdemethoxycucurmin (Standard) (Curcumin III (Standard)) is the analytical standard of Bisdemethoxycucurmin (HY-N0007A). This product is intended for research and analytical applications. Bisdemethoxycucurmin (Curcumin III) is a curcuminoid compound and an inhibitor of P-glycoprotein and ferroptosis. Bisdemethoxycucurmin exhibits multiple activities such as anti-oxidation, anti-inflammation and anti-tumor. Bisdemethoxycucurmin can be used for the research of tumors and inflammatory diseases.
    Bisdemethoxycucurmin (Standard)
  • HY-107372H
    UTP trisodium salt, 100 mM Solution, PCR Grade
    UTP (Uridine 5'-triphosphate) trisodium salt, 100 mM Solution, PCR Grade is a solution prepared from uridine triphosphate at a concentration of 100 mM, free of DNase, RNase and phosphatase contamination, suitable for molecular biology research. Uridine triphosphate (HY-107372) is a pyrimidine nucleoside triphosphate that is used as a substrate to synthesize RNA or as an energy source in metabolic reactions. Uridine triphosphate activates membrane-bound P2Y2 receptors.
    UTP trisodium salt, 100 mM Solution, PCR Grade
  • HY-14913
    Apricitabine
    		Inhibitor 99.8%
    Apricitabine (SPD754; AVX754), the (-) enantiomer of 2′-deoxy-3′-oxa-4′-thiocytidine (dOTC), is a highly selective and orally active HIV-1 reverse transcriptase (RT) inhibitor (Ki=0.08 μM), as well as inhibits DNA polymerases α, β, and γ with Ki value of 300 μM, 12 μM, and 112.25 μM, respectively. Apricitabine (SPD754; AVX754) shows promising antiretroviral efficacy, good tolerability and a low propensity for resistance selection in antiretroviral-naive HIV infection.
    Apricitabine
  • HY-B0003A
    1'-epi Gemcitabine hydrochloride
    		Control
    1'-epi Gemcitabine hydrochloride is the isomer of Gemcitabine hydrochloride (HY-B0003), and can be used as an experimental control. Gemcitabine Hydrochloride (LY 188011 Hydrochloride) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine Hydrochloride inhibits DNA synthesis and repair, resulting in autophagyand apoptosis.
    1'-epi Gemcitabine hydrochloride
  • HY-136597
    Remdesivir O-desphosphate acetonide impurity
    		Inhibitor 99.88%
    Remdesivir O-desphosphate acetonide impurity is an impurity of Remdesivir. Remdesivir (GS-5734), a nucleoside analogue with effective antiviral activity and is highly effective in the control of SARS-CoV-2 (COVID-19) infection in vitro.
    Remdesivir O-desphosphate acetonide impurity
  • HY-138561A
    EFdA-TP tetraammonium
    		Inhibitor 98.03%
    EFdA-TP tetraammonium is a potent nucleoside reverse transcriptase (RT) inhibitor. EFdA-TP tetraammonium inhibits RT-catalyzed DNA synthesis as an effective immediate or delayed chain terminator (ICT or DCT). EFdA-TP tetraammonium inhibits HIV-1 RT with multiple mechanisms. EFdA-TP (tetraammonium) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    EFdA-TP tetraammonium
  • HY-132146
    5-Propargylamino-ddCTP
    		Chemical
    5-Propargylamino-ddCTP, a nucleoside molecule that can be used to synthesis of cyanine dye-nucleotide conjugate which is used in nucleic acid labeling or sequence analysis.
    5-Propargylamino-ddCTP
  • HY-138561B
    EFdA-TP tetrasodium
    		Inhibitor
    EFdA-TP tetrasodium is a potent nucleoside reverse transcriptase (RT) inhibitor. EFdA-TP tetrasodium inhibits RT-catalyzed DNA synthesis as an effective immediate or delayed chain terminator (ICT or DCT). EFdA-TP tetrasodium inhibits HIV-1 RT with multiple mechanisms. EFdA-TP (tetrasodium) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
    EFdA-TP tetrasodium
  • HY-170873
    G3BP1/2-Targeting ligand-1
    		Ligand 99.61%
    G3BP1/2-Targeting ligand-1 is the ligand for G3BP1/2 that can be used as target protein ligand for synthesis of G3BP1/2 PROTAC degrader PT-129 (HY-170872).
    G3BP1/2-Targeting ligand-1
  • HY-50735
    Fiacitabine
    		Inhibitor 99.27%
    Fiacitabine (NSC 382097; FIAC; FOAC) is a potent and highly selective anti-herpesvirus agent. Fiacitabine acts as an inhibitor of HSV DNA polymerase, with a Ki of 0.26 μM for HSV-1 and 0.42 μM for HSV-2, respectively. Fiacitabine can be efficiently phosphorylated by thymidine kinase encoded by the virus itself to generate FIACTP, an active triphosphate metabolite. Fiacitabine is applicable to research related to herpesvirus infections.
    Fiacitabine
  • HY-W570886
    2'-O-MOE-U
    2'-O-MOE-U is a nucleic acid modification group (Phosphoramidite) with 3'-exonuclease inhibitory activity. 2'-O-MOE-U also exhibits gene silencing activity and double-stranded oligonucleotide stability. By forming steric interactions with 3'-exonuclease residues, 2'-O-MOE-U anchors the 3'-end of the siRNA guide strand in the hAgo2 PAZ domain, thereby regulating double-stranded thermal stability and enhancing base-pairing specificity. 2'-O-MOE-U does not induce IFNα production, can be incorporated at multiple sites of siRNA to enhance RNAi activity, and produces a synergistic effect with 2'-F modification. 2'-O-MOE-U has been widely used in studies related to breast cancer and other diseases.
    2'-O-MOE-U
  • HY-16516
    Vitamin CK3
    		Inhibitor 99.84%
    Vitamin CK3 is the combination of Vitamin C and vitamin K3 (100:1 ratio). Vitamin CK3 can decrease MMP activity. Vitamin CK3 can reactivate DNases and cause cell autophagy. Vitamin CK3 can be used for the research of cancer, such as lung cancer.
    Vitamin CK3
  • HY-134313A
    8-NH2-ATP tetrasodium
    		98.0%
    8-NH2-ATP tetrasodium, an inactive form of ATP, is produced by 8-NH2-Ado. 8-NH2-Ado tetrasodium induces apoptosis-related cleavage of poly (ADP-ribose) polymerase.
    8-NH2-ATP tetrasodium
  • HY-149442
    APE1-IN-3
    		Inhibitor
    APE1-IN-3 (Compound 1) is a APE1 inhibitor. APE1-IN-3 can be used for the research of cancer.
    APE1-IN-3
  • HY-101400S
    Deoxycytidine triphosphate-d14 dilithium
    		Activator
    Deoxycytidine triphosphate-d14 (dCTP-d14 dilithium; 2′-Deoxycytidine-5′-triphosphate-d14) dilithium is deuterium labeled Deoxycytidine triphosphate (HY-101400). Deoxycytidine triphosphate (dCTP) is a nucleoside triphosphate that can be used for DNA synthesis. Deoxycytidine triphosphate has many applications, such as real-time PCR, cDNA synthesis, and DNA sequencing.
    Deoxycytidine triphosphate-d<sub>14</sub> dilithium
  • HY-B0843AS
    Metalaxyl-M-d6
    		Inhibitor 99.9%
    Metalaxyl-M-d6 ((R)-Metalaxyl-d6) is the deuterium labeled Metalaxyl-M (HY-B0843A). Metalaxyl-M ((R)-Metalaxyl) is an orally active and selective inhibitor of fungal RNA polymerase, which exerts fungicidal activity by selectively interfering with the synthesis of fungal ribosomal RNA. Metalaxyl-M can also be used to induce inflammation in hepatocytes and regulate tryptophan metabolism. Metalaxyl-M can be used in ecotoxicology studies.
    Metalaxyl-M-d<sub>6</sub>
  • HY-W459577
    Zinc picolinate
    		Activator
    Zinc picolinate is an orally effective zinc supplement. Zinc picolinate reduces the expression of Hsp70, inhibits oxidative DNA damage, increases serum ALP activity, decreases MDA concentration, elevates SOD levels, and raises zinc concentrations in serum, whole body and plasma. Zinc picolinate does not alter copper and manganese contents in rainbow trout. Zinc picolinate exerts a protective effect against Acetic acid (HY-Y0319)-induced experimental colitis in Sprague Dawley rats. Zinc picolinate reduces uterine fibroid volume, alleviates colonic oxidative damage, relieves colonic inflammation, and enhances intestinal barrier integrity. Zinc picolinate can be used in research related to uterine fibroids, ulcerative colitis and chronic obstructive pulmonary disease.
    Zinc picolinate
Cat. No. Product Name / Synonyms Application Reactivity
DNA/RNA Synthesis Isoform Comparison

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